Dec. 12, 2016 - MIT hosted Journal Club where the EBICS community across 7 of its affiliated institutions and organizations tuned into Prof. Laurie Boyer's discussion of two papers "Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types" and "Tissue Mechanics Orchestrate Wnt-Dependent Human Embryonic Stem Cell Differentiation" to illustrate the importance of developmental engineering of cell fate decisions. In the context of EBICS, developmental engineering determines parameters that underpin generations of specific and homogeneous cell types, allows identification of key markers for quantifying in vitro emergent behavior in real time, and provides a critical roadmap, both for inferring synthetic circuits driving cell specification and for generating functional 3D-cell systems.
From "Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types," Boyer highlighted:
- Stepwise map of competing signals guiding mesoderm development
- Efficient human mesoderm induction by blocking formation of unwanted fates
- ESC-derived human bone progenitors and heart precursors engraft in vivo
- A transient segmentation program in human embryogenesis marked by HOPX
From "Tissue Mechanics Orchestrate Wnt-Dependent Human Embryonic Stem Cell Differentiation," Boyer highlighted:
- Compliant hydrogel substrates enhance mesoderm differentiation of human ESCs
- Stabilization of adherens junctions primes hESCs for mesoderm differentiation
- Junctional reorganization and Src activity promote nuclear translocation of b-catenin
- On stiff gels, b-catenin degradation inhibits mesodermal differentiation
Missed it? Listen to the discussion now!